Dr. Hisham Bazzi

Research Area: Mouse Embryonic Development & Skin Development and Patterning

Website:  cecad.uni-koeln.de/Dr-Hisham-Bazzi.422.0.html

1. Research Background:

In order to understand aging and how the organism is disassembled, we need to understand how it was built in the first place! Our main interest is to study embryonic development and skin development and patterning in the mouse.

There are three main projects in the lab.

  1. Studying the roles of centrosomes, the major microtubule organizing centers in animal cells, in mouse embryonic development and skin epidermal development.
  2. Deciphering the roles of a new protein complex called STRIPAK in cellular adhesion and migration in the same developmental contexts.
  3. Investigating the mechanisms of hair follicle initiation and patterning in the mouse skin.

2. Research questions addresses by the group:

  1. a) How does the disruption of centrosomes lead to a novel p53-checkpoint activation?
    b) How do centrosomes regulate mitotic spindle orientation and cell fate?
    c) What distinct processes and signaling pathways do centrosomes and cilia regulate?
  2. How does the STRIPAK complex regulate cell adhesion and migration?
  3. How are hair follicles initiated and patterned in the skin?

3. Possible projects:

  • How does the STRIPAK complex regulate cell adhesion and migration in the skin dermis?
  • How are hair follicles initiated and patterned in the skin epithelium?

4. Applied Methods and model organisms:

Our models are the mouse and mouse-derived cells: embryonic stem cells (mESCs), skin epidermal keratinocytes and fibroblasts.

Methods include:

  • Generating knockout and knock-ins using CRISPR/Cas9 in mESCs and in vivo
  • Live imaging of cells, embryos and skin explants
  • RNA-Seq, ChIP-Seq, scRNA-Seq and Mass Spec
  • Histology, immunofluorescence and immunohistochemistry
  • Transmission and scanning electron microscopy
  • Biochemical techniques (such as Western Blots and IPs)
  • Molecular cloning

5. Desirable skills and qualifications:

Passion for developmental biology

6. References:

  • Bazzi H & Anderson KV. (2014). Centrioles in the mouse: cilia and beyond. Cell Cycle, 13, 2809.
  • Insolera R*, Bazzi H*, Shao W, Shi SH, Anderson KV. (2014). Cortical neurogenesis in the absence of centrioles. Nat Neurosci, 17, 1528-35.
  • Bazzi H & Anderson KV. (2014). Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc Natl Acad Sci U S A, 111 , E1491-500.
  • Bazzi H, Fantauzzo KA, Richardson GD, Jahoda CA & Christiano AM. (2007). The Wnt inhibitor, Dickkopf 4, is induced by canonical Wnt signaling during ectodermal appendage morphogenesis. Dev Biol, 305, 498-507.
  • Kljuic A, Bazzi H, et al. (2003). Desmoglein 4 in hair follicle differentiation and epidermal adhesion: evidence from inherited hypotrichosis and acquired pemphigus vulgaris. Cell, 113, 249-60.